Fish Analysis for Drug and Chemicals Mediated Cellular Toxicity by Ajit Kumar Saxena & Amit Kumar

Author:Ajit Kumar Saxena & Amit Kumar
Language: eng
Format: epub
ISBN: 9789811547003
Publisher: Springer Singapore

4.2 Experimental Procedure

4.2.1 Materials

The testis of 1 day of old pups was collected from nine parallel experimental sets in which mothers were exposed (i.p.) with cyclophosphamide (i.e., single dose of 2 or 10 or 20 mg/kg) either on 12th or 15th or 18th day of gestation period. The testes were fixed in Bouin’s solutions for 24 h. After fixation, the testes were dehydrated with ascending grade of alcohol and cleared in xylene. The testes were impregnated with paraffin wax (melting point, 56°–58°). The transverse sections of 7 μm were used after staining with hematoxylin and eosin.

Hematoxylin (Ehrlich’s) Absolute alcohol

33 ml

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